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For this, three groups of 10 rats were administered with normal saline (NS), VSL#3 (1 × 108 CFU), or marine algae extract (MAE; 1 g/kg bw/day) for 14 days. After 14 days, rats were exposed to an established forced swimming test (FST), and immobility time was recorded. The rats were then euthanized, and the prefrontal cortex (PFC) and hippocampus were isolated and processed for histopathological examinations. Dietary supplementation of VSL#3 or MAE significantly increased the time spent in active swimming and decreased the duration of immobility in FST compared to that of the NS-treated group. In the PFC, MAE significantly increased neuronal density, while no significant change was observed in VSL#3-treated rats. In the hippocampus, a significant increase was observed in neuronal density in both VSL#3- and MAE-treated rats. Moreover, in VSL#3-treated rats, the cell density was significantly increased in the CA1 and CA3 regions of the hippocampus. 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